533 Active ingredient from Bombax costatum (kapok tree) to preserve skin microbiota equilibrium

Preserving skin microbiota homeostasis is of crucial importance for maintaining healthy skin. We have developed a new active ingredient from Bombax costatum flowers, this polysaccharide-rich extract was evaluated in vitro on models using different bacterial strains representative cutaneous (S. aureus, S. epidermidis, hominis, C. acnes). The significantly increased keratinocyte production innate immunity markers TLR2 (Toll-Like Receptor 2) and hBD2 (human beta-Defensin by ELISA assay. In co-culture several strains, the tended to inhibit growth pathogenic strain without affecting commensal bacteria. This effect even more significant as aureus present high proportions compared other suggesting preventive toward pathogenicity limiting its overgrowth. inhibited biofilm formation, crystal violet dye, while it that epidermidis. adhesion reconstructed human epidermis (RHE) adhesion. Adhesion colony forming unit counting adhering bacteria after topical pre-treatment extract. Finally, protected expression filaggrin, desmoglein-1 corneodesmosin, assessed immunostaining RHE were incubated presence secretum. demonstrated natural plant obtained able limit growth, properties aureus), Moreover, stimulates immune defenses helps preserve epidermal barrier alteration due aureus. These results show beneficial homesostasis promoting against strains.